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Catalog Number	Size	Price
LS-C54629-100	100 µg (0.5 mg/ml)	$503

CD274 / B7-H1 / PD-L1 Antibody - CD274 / PD-L1 Antibody Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

CD274 / B7-H1 / PD-L1 Antibody - CD274 / PD-L1 Antibody (2µg/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

CD274 / B7-H1 / PD-L1 Antibody - CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

CD274 / B7-H1 / PD-L1 Antibody - CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

CD274 / B7-H1 / PD-L1 Antibody - CD274 / B7-H1 / PD-L1 antibody (0.3µg/ml) staining of Human Heart (A) lysate + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.

CD274 / B7-H1 / PD-L1 Antibody - Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

CD274 / B7-H1 / PD-L1 Antibody - CD274 / B7-H1 / PD-L1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Polyclonal Goat anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (aa166‑176, IHC, IF, WB) LS‑C54629

Name: Anti-CD274 / B7-H1 / PD-L1 Antibody | Goat anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C54629-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

CD274 / B7-H1 / PD-L1 Goat anti-Human Polyclonal (aa166-176) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$503

LS-C54629-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD274 / B7-H1 / PD-L1 Goat anti-Human Polyclonal (aa166-176) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PD-L1 antibody LS-C54629 is an unconjugated goat polyclonal antibody to human PD-L1 (CD274 / B7-H1) (aa166-176). Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human CD274 / B7-H1 / PD-L1

Synonyms

CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1

See All CD274 / B7-H1 / PD-L1 Antibodies

Host

Goat

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence CKKQSDTHLEET, from the C-Terminus of protein sequence according to NP_054862.1NP_001254635.1.

Epitope

aa166-176

Specificity

Human CD274.

Applications

IHC
IHC - Paraffin (4 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (0.03 - 0.1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD274 / B7-H1 / PD-L1

Uniprot: Q9NZQ7 NCBI: NM_014143 NP_054862.1