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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD27 Antibody (clone O323, PE, Cy7) LS‑C107367
CD27 antibody LS-C107367 is a PE, Cy7-conjugated mouse monoclonal antibody to human CD27. Validated for Flow.
Catalog
Size
Price
LS-C107367-25
25 tst
Unavailable
LS-C107367-100
100 tst
Unavailable

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Applications: IHC, Immunoprecipitation, Flow Cytometry

Product Description

CD27 antibody LS-C107367 is a PE, Cy7-conjugated mouse monoclonal antibody to human CD27. Validated for Flow.
About CD27
CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. P26842 NM_001242 NP_001233.1

CD27 Antibody, CD27 antigen Antibody, CD27 molecule Antibody, S152 Antibody, T14 Antibody, T-cell activation antigen CD27 Antibody, CD27L receptor Antibody, T cell activation antigen S152 Antibody, TNFRSF7 Antibody, Tp55 Antibody


Specifications

Target
Human CD27
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [O323]
Conjugations
Phycoerythrin, Cy7
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
CD27 antibody was raised against human CD27
Usage
This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 ul (0.25 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The pre-titrated test size contains BSA and/or gelatin for protein stabilization. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, <=0.09% sodium azide, protein stabilizer
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K isotype control (open histogram) or PE-Cy7 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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