Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone LT27] (IgG2a) to Human CD27
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [LT27]
Ion exchange chromatography
IHC - Paraffin
Specificity and Use
CD27 antibody was raised against human peripheral blood lymphocytes.
Reacts with CD27 (T14), a 50-55 kD type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
Flow Cytometry: Recommended dilution - 2 ug/ml.
PBS, 15 mM sodium azide, approx., pH 7.4.
+4°C or -20°C, Avoid repeated freezing and thawing.
CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor.