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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3120-50 | 50 µg (0.1 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Hamster anti‑Mouse CD27 Antibody (clone LG.3A10, FITC, IHC) LS‑B3120
IHC‑plus™ Monoclonal Hamster anti‑Mouse CD27 Antibody (clone LG.3A10, FITC, IHC) LS‑B3120
Note: This antibody replaces LS-C62945
Antibody:
CD27 Hamster anti-Mouse Monoclonal (FITC) (LG.3A10) Antibody
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Mouse, Human
Format:
FITC, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD27 Hamster anti-Mouse Monoclonal (FITC) (LG.3A10) Antibody
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Mouse, Human
Format:
FITC, Unmodified
Specifications
Description
CD27 antibody LS-B3120 is an FITC-conjugated hamster monoclonal antibody to CD27 from mouse. It is reactive with human and mouse. Validated for Flow and IHC.
Target
Mouse CD27
Synonyms
CD27 | CD27 antigen | CD27 molecule | S152 | T14 | T-cell activation antigen CD27 | CD27L receptor | T cell activation antigen S152 | TNFRSF7 | Tp55
Host
Hamster
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
LG.3A10
Conjugations
FITC
Purification
Protein G purified from ascites
Modifications
Unmodified
Immunogen
MCD27 tag expressing clones AR0524 and AR0530
Specificity
Anti-mouse CD27 Monoclonal Antibody is specific for the mouse CD27 receptor.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Flow Cytometry (0.5 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3120 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide, BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD27
Validation
Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ CD27 Antibody (clone LG.3A10, FITC) for IHC, Flow LS-B3120 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Frozen, ICC, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
