Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor.
Western Blot analysis of TNFRSF7 expression in transfected 293T cell line by TNFRSF7 monoclonal antibody (M01), clone 1E2-A3.Lane 1: TNFRSF7 transfected lysate(29.2 KDa).Lane 2: Non-transfected lysate.
TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 Western Blot analysis of TNFRSF7 expression in Jurkat.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CD27 is 0.3 ng/ml as a capture antibody.
Western blot analysis of TNFRSF7 over-expressed 293 cell line, cotransfected with TNFRSF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 . GAPDH ( 36.1 kDa ) used as specificity and loading control.