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Catalog Number Size Price
LS-C662908-0.1 0.1 mg (1 mg/ml) $302 
CD247 / CD3 Zeta Antibody - Cell lyzates of HEK293T/17 cells transiently transfected  with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54).    1: Wt mCD3zeta pervanadate non-stimulated  2: Wt mCD3zeta pervanadate  stimulated  3: mut. mCD3zeta Y/F2-6  4: mut. mCD3zeta Y/F1 and 3-6  5: mut. mCD3zeta Y/F1-2 and 4-6  6: mut. mCD3zeta Y/F1-3 and 5-6  7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)  8: mut. mCD3zeta Y/F1-5

Monoclonal Mouse anti‑Human CD247 / CD3 Zeta Antibody (clone EM‑54, phospho‑Tyr142, WB) LS‑C662908

Monoclonal Mouse anti‑Human CD247 / CD3 Zeta Antibody (clone EM‑54, phospho‑Tyr142, WB) LS‑C662908

Antibody:
CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr142) (EM-54) Antibody
Application:
WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$302
LS-C662908-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr142) (EM-54) Antibody
Application:
WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CD3 Zeta antibody LS-C662908 is an unconjugated mouse monoclonal antibody to CD3 Zeta (CD247) (pTyr142) from human. It is reactive with human and mouse. Validated for Flow and WB.
Target
Human CD247 / CD3 Zeta
Synonyms
CD247 | CD3 Zeta | CD3H | CD3Z | CD247 antigen, zeta subunit | CD247 molecule | CD3zeta chain | T3Z | TCR zeta chain | TCRZ | T-cell receptor T3 zeta chain | CD247 antigen | CD3-ZETA | CD3Q
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
EM-54
Conjugations
Unconjugated
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Epitope
pTyr142
Specificity
The mouse monoclonal antibody EM-54 recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Applications
  • Western blot
  • Flow Cytometry
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD247 / CD3 Zeta
P20963 NM_000734 NP_000725.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024