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Anti-CD247 / CD3 Zeta Antibody (clone 4B10) IHC-plus™ LS-B4145

Note: This antibody replaces LS-C109131, LS-C171332

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD247 / CD3 Zeta Antibodies

Anti-CD247 / CD3 Zeta Antibody (clone 4A12-F6) IHC-plus™ LS-B6021
Mouse Monoclonal [clone 4A12-F6] (IgG1,k) to Human CD247 / CD3 Zeta
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4B10] (IgG1) to Human CD247 / CD3 Zeta
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human CD247 / CD3 Zeta
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4B10]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA

Specificity and Use

CD247 / CD3 Zeta antibody was raised against purified recombinant fragment of human CD247 expressed in E. coli.
Human CD247

Packaging

Ascitic fluid, 0.03% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CD247 / CD3 Zeta

P20963 NM_000734 NP_000725.1

CD247 Antibody, CD3 Zeta Antibody, CD3H Antibody, CD3Z Antibody, CD247 antigen, zeta subunit Antibody, CD247 molecule Antibody, CD3zeta chain Antibody, T3Z Antibody, TCR zeta chain Antibody, TCRZ Antibody, T-cell receptor T3 zeta chain Antibody, CD247 antigen Antibody, CD3-ZETA Antibody, CD3Q Antibody

CD247 / CD3 Zeta is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-CD247 / CD3-Zeta antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4145 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-CD247 / CD3-Zeta antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4145 dilution 1:200.

Immunofluorescence

Immunofluorescence
Figure3: Immunofluorescence of K562 cells using anti-CD247 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using CD247 monoclonal antibody against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.

Requested From: United States
Date Requested: 12/4/2016

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