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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD247 / CD3 Zeta Antibody (aa2‑31) LS‑C98114
CD3 Zeta antibody LS-C98114 is an unconjugated rabbit polyclonal antibody to human CD3 Zeta (CD247). Validated for Flow and WB.
Catalog
Size
Price
LS-C98114-400
400 µl
Unavailable

Popular CD247 / CD3 Zeta Products

Antibody
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA
Anti-CD247 / CD3-Zeta antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6021 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunofluorescence analysis of A549 cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Western blot analysis of CD247 expression in Jurkat (A); NS-1 (B); PC12 (C) whole cell lysates.
Species: Human, Monkey, Mouse, Rat, Pig, Rabbit
Applications: Western blot

Product Description

CD3 Zeta antibody LS-C98114 is an unconjugated rabbit polyclonal antibody to human CD3 Zeta (CD247). Validated for Flow and WB.
About CD247 / CD3 Zeta
CD247 / CD3 Zeta is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. P20963 NM_000734 NP_000725.1

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Specifications

Target
Human CD247 / CD3 Zeta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E3008 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98114. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-31
Specificity
This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human CD3Z.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.
Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.
Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.
Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.
Western blot of anti-CD3Z Antibody (RB13817) in Ramos cell line lysates (35 ug/lane). CD3Z(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of Ramos cells using CD3Z Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/24/2018

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