Mouse CD244

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

NS0-derived rm2B4 extracellular domain.

Extracellular Domain

Recognizes mouse 2B4 extracellular domain.

• IHC
• Immunofluorescence
• Western blot
• Flow Cytometry
• ELISA

Direct ELISA, Western Blot, Flow Cytometry, Immunohistochemistry. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 0.3 ng/well. In this format, this antibody shows approximately 5% cross-reactivity with rh2B4 and less than 1% cross-reactivity with rhSLAM. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 ug/mL/1E6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Immunohistochemistry: This antibody will detect 2B4 in cells and tissues. The working dilution is 5-15 ug/mL.

Lyophilized from PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BZW8 NCBI: NM_016382 NP_057466.1