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Anti-CD24 Antibody (aa27-59) LS-C149903

Catalog Size Price
LS-C149903-25 25 µg Unavailable
LS-C149903-100 100 µg Unavailable

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100% Guaranteed
Sheep Polyclonal (IgG) to Human CD24
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CD24
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

CD24 antibody was raised against chinese hamster ovary cell line CHO-derived recombinant human CD24 Ser27-Gly59 Accession # NP_037362.
aa27-59
Detects human CD24 in direct ELISAs and Western blots. In direct ELISAs, less than 1 % cross-reactivity with recombinant mouse CD24a is.
Western Blot, 1 µg/ml. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human colon cancer tissue and lymph node. tissue.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CD24

NM_013230 NP_037362.1

CD24 Antibody, CD24 molecule Antibody, CD24A Antibody, Signal transducer CD24 Antibody, CD24 antigen Antibody

CD24 is a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y.

Western blot

Western blot
Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 ug/ml of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for CD24 at approximately 42 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: 
Date Requested: 12/18/2017

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