Human CD209 / DC-SIGN

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NIH/3T3 transfected with recombinant, human DC-SIGN.

Recognizes human DC-SIGN on transfected NIH/3T3 cells and on monocyte derived dendritic cells at 44kD. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

• IHC (25 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry
• ELISA

Suitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Flow Cytometry (Immunophenotyping): 10 ul of 25-50 ug/ml labels up to 10^6 cells. Adhesion Blockade: 5 ug/ml (5x10^6 cells/well). Immunohistochemistry: 25 ug/ml detects human DC-SIGN in fixed cells and tissue sections. Western Blot: 1-2 ug/ml The detection limit is a ~5 ng/well under non-reducing and reducing conditions using a colorimetric detection system,. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Lyophilized from in PBS, pH 7.2, 5% trehalose

Sterile 40-50% glycerol, PBS.

Lyophilized powder may be stored at -20°C for short-term only. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NNX6 NCBI: NM_021155 NP_066978.1