Human CD209 / DC-SIGN

Rat

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

h209

Unconjugated

Affinity purified

Unmodified

Human CD209

• IHC
• IHC - Paraffin (20 µg/ml)
• Immunoprecipitation
• Flow Cytometry

Immunohistochemistry: LS-B3782 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3782 was determined to be 20 ug/ml. Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NNX6 NCBI: NM_021155 NP_066978.1