Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone MEM-97] (IgG1) to Human CD20
Human, Bovine, Pig
Immunoprecipitation, Flow Cytometry
Human, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MEM-97]
Protein A purified
IHC - Paraffin
Specificity and Use
CD20 antibody was raised against raji human Burkitt's lymphoma cell line.
Reacts with CD20 (Bp35), a 33-37 kD non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Flow Cytometry: Recommended dilution - 10 ug/ml.
PBS, 15 mM sodium azide, approx., pH 7.4.
+4°C or -20°C, Avoid repeated freezing and thawing.
CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.
Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control (PPV-06) Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97)
Requested From: United States
Date Requested: 10/22/2016