Rat CD1D

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

WTH1

FITC

Protein A purified

Unmodified

Rat CD1d-transduced M12.4.1. C3 cells.

Recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines. Clone WTH1 recognizes a different, non-overlapping epitope than clone WTH2 (, or ), and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2. Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.

• Flow Cytometry (1:1000)

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15813 NCBI: NM_001766 NP_001757.1