Target
Human CD19
Synonyms
CD19 | B-lymphocyte antigen CD19 | CD19 molecule | Differentiation antigen CD19 | T-cell surface antigen Leu-12 | B4 | CD19 antigen | CVID3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-ENDSNLGQDQLS, from the internal region of the protein sequence according to NP_001171569.1NP_001761.3.
Specificity
Human CD19. This antibody is expected to recognize both reported isoforms (NP_001171569.1; NP_001761.3).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kD according to NP_NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee