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Anti-CD19 Antibody (aa393-421) IHC-plus™ LS-B3624

Note: This antibody replaces LS-C98254
Catalog Size Price
LS-B3624-200 200 µl Unavailable

Related Products

248 CD19 Antibodies

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Anti-CD19 Antibody (aa393-421) IHC-plus™ LS-B3624
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IHC - Paraffin, Western blot
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Immunohistochemistry Image
Anti-CD19 Antibody LS-C122956
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Anti-CD19 Antibody (C-Terminus, clone SP110) IHC-plus™ LS-B11667
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Anti-CD19 Antibody (C-Terminus) IHC-plus™ LS-B13077
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100% Guaranteed
Rabbit Polyclonal to Human CD19
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CD19
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)

Specificity and Use

LS-E3739 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3624. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa393-421
This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-421 amino acids from the C-terminal region of human CD19.
Immunohistochemistry: LS-B3624 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3624 was determined to be 5 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CD19

P15391 NM_001770 NP_001761.3

CD19 Antibody, B-lymphocyte antigen CD19 Antibody, CD19 molecule Antibody, Differentiation antigen CD19 Antibody, T-cell surface antigen Leu-12 Antibody, B4 Antibody, CD19 antigen Antibody, CVID3 Antibody

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Immunohistochemistry

Immunohistochemistry
Anti-CD19 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3624 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CD19 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3624 concentration 5 ug/ml.

Western blot

Western blot
Western blot of lysates from Raji, Ramos cell line (from left to right), using CD19 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of lysates from Raji, Ramos cell line (from left to right), using CD19 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Requested From: 
Date Requested: 12/15/2017

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