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Anti-CD177 Antibody (clone MEM-166) IHC-plus™ LS-B1953

Note: This antibody replaces LS-C40845

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD177 Antibodies

Anti-CD177 Antibody (clone MEM-166) IHC-plus™ LS-B1953
Mouse Monoclonal [clone MEM-166] (IgG1) to Human CD177
Human, Monkey
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD177 Antibody (clone 4C4) IHC-plus™ LS-B5469
Mouse Monoclonal [clone 4C4] (IgG2a,k) to Human CD177
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CD177 Antibody (aa193-468) LS-C295622
Rabbit Polyclonal (IgG) to Mouse CD177
Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone MEM-166] (IgG1) to Human CD177
Human, Monkey
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CD177
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MEM-166]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1 µg/10E6 cells)

Specificity and Use

CD177 antibody was raised against cells.
Human granulocytes
Immunohistochemistry: LS-B1953 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1953 was determined to be 10 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
+4°C. Store undiluted.
For research use only.

Publications (1)

1
Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. Pradhan Nabzdyk L, Kuchibhotla S, Guthrie P, Chun M, Auster ME, Nabzdyk C, Deso S, Andersen N, Gnardellis C, LoGerfo FW, Veves A. Journal of vascular surgery. 2013 Sep;58:766-75.e12. [Full Text Article] [PubMed:23755976] [PMC:PMC3755034]

About CD177

Q8N6Q3 NM_020406 NP_065139.2

CD177 Antibody, CD177 molecule Antibody, CD177 antigen Antibody, Cell surface receptor Antibody, HNA-2a Antibody, HNA2A Antibody, NB1 Antibody, PRV-1 Antibody, Polycythemia rubra vera 1 Antibody, PRV1 Antibody, NB1 glycoprotein Antibody, NB1 GP Antibody

CD177 encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera.

Immunohistochemistry

Immunohistochemistry
Anti-CD177 antibody IHC of human intravascular neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1953 concentration 10 ug/ml.

Flow Cytometry

Flow Cytometry
Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC

Requested From: United States
Date Requested: 12/9/2016

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