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Anti-CD163 Antibody (clone GHI/61) IHC-plus™ LS-B3781

Note: This antibody replaces LS-C106905

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD163 Antibodies

Anti-CD163 Antibody (clone 10D6) LS-C87534
Mouse Monoclonal [clone 10D6] (IgG1) to Human CD163
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-CD163 Antibody (clone GHI/61) IHC-plus™ LS-B3781
Mouse Monoclonal [clone GHI/61] (IgG1,k) to Human CD163
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD163 Antibody (clone ED2) IHC-plus™ LS-B5967
Mouse Monoclonal [clone ED2] (IgG1) to Rat CD163
Rat
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone GHI/61] (IgG1,k) to Human CD163
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human CD163
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [GHI/61]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot
  • Flow Cytometry

Specificity and Use

CD163 antibody was raised against spleen cells from a case of hairy cell leukemia
CD163
Immunohistochemistry: LS-B3781 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3781 was determined to be 20 ug/ml. Tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About CD163

Q86VB7 NM_004244 NP_004235.4

CD163 Antibody, CD163 antigen Antibody, Macrophage-associated antigen Antibody, M130 Antibody, CD163 molecule Antibody, Hemoglobin scavenger receptor Antibody, MM130 Antibody

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner.

Immunohistochemistry

Immunohistochemistry
Anti-CD163 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3781 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CD163 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3781 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CD163 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3781 concentration 20 ug/ml.

Flow Cytometry

Flow Cytometry
Staining of normal human peripheral blood cells with 0.25 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 ug of Purified anti-human CD163 (GHI/61) (colored histogram) followed by FITC Anti-Mouse IgG. Cells in the monocyte gate were used for analysis.

Requested From: United States
Date Requested: 12/6/2016

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