Human CD14

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

TÜK4

Unconjugated

Protein G purified

Low Endotoxin

Recognizes the human CD14 cell surface antigen. CD14 is a 55 KDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage but a soluble form of CD14 also exists. CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells. CD14 functions as a pattern recognition receptor, in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. Has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner.

• Western blot
• Flow Cytometry (1:100 - 1:200)
• ELISA

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08571 NCBI: NM_000591 NP_000582.1