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Anti-CD135 / FLT3 Antibody (Tyr591) LS-C150278

Ordering

Wt. Vol. Conc. Price
50 µg - - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD135 / FLT3 Antibodies

Anti-CD135 / FLT3 Antibody (Internal) IHC-plus™ LS-A7148
Rabbit Polyclonal to Human CD135 / FLT3
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CD135 / FLT3 Antibody (clone BV10A4H2) IHC-plus™ LS-B4049
Mouse Monoclonal [clone BV10A4H2] (IgG1) to Human CD135 / FLT3
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD135 / FLT3 Antibody IHC-plus™ LS-A7147
Rabbit Polyclonal to Human CD135 / FLT3
Human, Monkey, Dog, Horse, Pig, Rabbit
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CD135 / FLT3
Human
ICC, Western blot
Unconjugated

Details

Human CD135 / FLT3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)

Specificity and Use

CD135 / FLT3 antibody was raised against phosphopeptide containing human Flt3 Y591 site.
Tyr591
Detects human Flt3 when phosphorylated at Y591.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 250 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CD135 / FLT3

P36888 NM_004119 NP_004110.2

FLT3 Antibody, CD135 Antibody, CD135 antigen Antibody, Fetal liver kinase 2 Antibody, Fetal liver kinase-2 Antibody, FL cytokine receptor Antibody, FLK2 Antibody, FLT-3 Antibody, Fms-like tyrosine kinase 3 Antibody, Stem cell tyrosine kinase 1 Antibody, STK-1 Antibody, FLK-2 Antibody, Fms-related tyrosine kinase 3 Antibody, STK1 Antibody

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Immunocytochemistry

Immunocytochemistry
Phospho-Flt-3/Flk-2 (Y591) in A431 Human Cell Line. Flt-3/Flk-2 phosphorylated at Y591 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho Flt-3/Flk-2 (Y591) Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human Phospho-Flt-3/Flk-2 (Y591) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 uM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti- Human Phospho-Flt-3/Flk-2 (Y591) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. Specific bands were detected for Phospho Flt-3/Flk-2 (Y591) at approximately 160 and 130 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/10/2016

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