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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD135 / FLT3 Antibody (Internal) IHC‑plus™ LS‑A7148
Note: This antibody replaces LS-C66202, LS-C42450
FLT3 antibody LS-A7148 is an unconjugated rabbit polyclonal antibody to human FLT3 (CD135). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A7148-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

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Product Description

FLT3 antibody LS-A7148 is an unconjugated rabbit polyclonal antibody to human FLT3 (CD135). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About CD135 / FLT3
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. P36888 NM_004119 NP_004110.2

FLT3 Antibody, CD135 Antibody, CD135 antigen Antibody, Fetal liver kinase 2 Antibody, Fetal liver kinase-2 Antibody, FL cytokine receptor Antibody, FLK2 Antibody, FLT-3 Antibody, Fms-like tyrosine kinase 3 Antibody, Stem cell tyrosine kinase 1 Antibody, STK-1 Antibody, FLK-2 Antibody, Fms-related tyrosine kinase 3 Antibody, STK1 Antibody


Specifications

Target
Human CD135 / FLT3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (8 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD135 / FLT3 antibody was raised against synthetic 20 amino acid peptide from internal region of human FLT3 / CD135. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rabbit (95%); Hamster (85%); Bovine, Dog, Panda, Horse (80%).
Blocking Peptide
LS-E29232 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A7148. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human FLT3 / CD135. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A7148 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A7148 was determined to be 8 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 7/20/2018

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