Target
Human CD121b / IL1R2
Synonyms
IL1R2 | CD121b | CD121b antigen | CDw121b | IL-1RT-2 | IL1R2c | Interleukin-1 receptor beta | Interleukin-1 receptor type II | IL-1R-beta | Antigen CDw121b | IL-1 type II receptor | IL-1R-2 | IL-1RT2 | IL1RB | Interleukin-1 receptor type 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QYNITRSIELRIKK, from the internal region of the protein sequence according to NP_004624.1NP_001248348.1.
Specificity
Human IL1R2. Reported variants represent identical protein: NP_775465.1, NP_004624.1
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Preliminary experiments gave an approx 38kDa band in Human Spleen lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.4kDa according to the precursor NP_004624.1 and 43.8kDa for the mature protein. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee