Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 8A1] (IgG2b) to Human CD105
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Rabbit Polyclonal (IgG) to Mouse CD105
Western blot, ELISA
Mouse (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
CD105 antibody was raised against synthetic peptide (KLH- coupled) corresponding to residues surrounding Pro430 of mouse endoglin.
Recognizes transfected levels of mouse endoglin protein at 81 kD.
Suitable for use in ELISA and Western Blot. Recommended Antibody Dilutions: Western blot: 1:1000 incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
CD105 is a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer.