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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCT2 / CCT Beta Antibody (clone PK/8/4/4i/2f) IHC‑plus™ LS‑B3740
Note: This antibody replaces LS-C63150
CCT Beta antibody LS-B3740 is an unconjugated rat monoclonal antibody to CCT Beta (CCT2) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3740-50
50 µg (0.34 mg/ml)
Unavailable

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Product Description

CCT Beta antibody LS-B3740 is an unconjugated rat monoclonal antibody to CCT Beta (CCT2) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CCT2 / CCT Beta
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. P78371 NM_006431 NP_006422.1

CCT2 Antibody, 99D8.1 Antibody, CCT-beta Antibody, CCTB Antibody, PRO1633 Antibody, TCP-1-beta Antibody, TCP1 Beta Antibody


Specifications

Target
Mouse CCT2 / CCT Beta
Host
Rat
Reactivity
Mouse, Human, Monkey, Rat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [PK/8/4/4i/2f]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CCT2 / CCT Beta antibody was raised against mouse CCT-beta peptide-PPD conjugate.
Specificity
Detects an ~55 kD protein, corresponding to the apparent molecular mass of TCP-1beta on SDS-PAGE immunoblots. This antibody does not react with human Hsp60 nor with E. coli GroEL proteins.
Usage
Immunohistochemistry: LS-B3740 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3740 was determined to be 10 ug/ml.
Presentation
PBS 10 mM sodium phosphate, pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Immunohistochemistry

Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Western blot

Western blot of human Jurkat cell lysate.
Western blot of human Jurkat cell lysate.

Immunohistochemistry

Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Immunohistochemistry

Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Western blot

Western blot of human Jurkat cell lysate.
Western blot of human Jurkat cell lysate.

Immunohistochemistry

Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Immunohistochemistry

Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Western blot

Western blot of human Jurkat cell lysate.
Western blot of human Jurkat cell lysate.

Immunohistochemistry

Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Immunohistochemistry

Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.
Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Western blot

Western blot of human Jurkat cell lysate.
Western blot of human Jurkat cell lysate.


Requested From: 
Date Requested: 6/22/2018

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