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Anti-CCT2 / CCT Beta Antibody (clone PK/8/4/4i/2f) IHC-plus™ LS-B3740

Note: This antibody replaces LS-C63150

Ordering

Wt. Vol. Conc. Price
50 µg - 0.34 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCT2 / CCT Beta Antibodies

Anti-CCT2 / CCT Beta Antibody (clone F39 P7 F11) IHC-plus™ LS-B1661
Mouse Monoclonal [clone F39 P7 F11] (IgG2a) to Human CCT2 / CCT Beta
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CCT2 / CCT Beta Antibody (clone PK/8/4/4i/2f) IHC-plus™ LS-B3740
Rat Monoclonal [clone PK/8/4/4i/2f] (IgG2b) to Mouse CCT2 / CCT Beta
Mouse, Human, Monkey, Rat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CCT2 / CCT Beta Antibody IHC-plus™ LS-B4861
Rabbit Polyclonal to Human CCT2 / CCT Beta
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone PK/8/4/4i/2f] (IgG2b) to Mouse CCT2 / CCT Beta
Mouse, Human, Monkey, Rat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse CCT2 / CCT Beta
Rat
Mouse, Human, Monkey, Rat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [PK/8/4/4i/2f]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

CCT2 / CCT Beta antibody was raised against mouse CCT-beta peptide-PPD conjugate.
Detects an ~55 kD protein, corresponding to the apparent molecular mass of TCP-1beta on SDS-PAGE immunoblots. This antibody does not react with human Hsp60 nor with E. coli GroEL proteins.
Immunohistochemistry: LS-B3740 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3740 was determined to be 10 ug/ml.

Packaging

PBS 10 mM sodium phosphate, pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
For research use only.

About CCT2 / CCT Beta

P78371 NM_006431 NP_006422.1

CCT2 Antibody, 99D8.1 Antibody, CCT-beta Antibody, CCTB Antibody, PRO1633 Antibody, TCP-1-beta Antibody, TCP1 Beta Antibody

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

Immunohistochemistry

Immunohistochemistry
Anti-CCT2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CCT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3740 concentration 10 ug/ml.

Western blot

Western blot
Western blot of human Jurkat cell lysate.
 

Requested From: United States
Date Requested: 12/5/2016

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