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Anti-CCR7 Antibody LS-C78053

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCR7 Antibodies

Anti-CCR7 Antibody (C-Terminus) IHC-plus™ LS-A848
Rabbit Polyclonal to Human CCR7
Human, Monkey, Bat, Bovine, Horse
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2a) to Mouse CCR7
Mouse
IHC, Flow Cytometry, Functional Assay, Neutralization
Unconjugated

Details

Mouse CCR7
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC (25 µg/ml)
  • Flow Cytometry
  • Functional Assay
  • Neutralization

Specificity and Use

CCR7 antibody was raised against rBC-2H3 cells expressing mCCR7.
Recognizes mouse CCR7.
Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 ug/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 ug/mL in the presence of 25 ng/mL of rmMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.

Packaging

Lyophilized from PBS, pH 7.4, 5% trehalose
200 µl Sterile PBS
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CCR7

P32248 NM_001838 NP_001829.1

CCR7 Antibody, BLR2 Antibody, Chemokine (C-C) receptor 7 Antibody, CD197 Antibody, C-C CKR-7 Antibody, CMKBR7 Antibody, CC chemokine receptor 7 Antibody, CCR-7 Antibody, CD197 antigen Antibody, CDw197 Antibody, EBI1 Antibody, EVI1 Antibody, MIP-3 beta receptor Antibody, C-c chemokine receptor 7 Antibody, C-C chemokine receptor type 7 Antibody, CC-CKR-7 Antibody, Chemokine c-c motif receptor 7 Antibody

CCR7, a Chemokine Receptor, is involved in lymphocyte recirculation through secondary lymphoid organs and moving lymphocytes and dendritic cells into distinct tissue compartments. CCR7 is called the Epstein-Barr-induced receptor because it mediates Epstein-Barr viral effects on B lymphocytes. CCR7 expression is up-regulated in mature dendritic cells, B cells infected with EBV, and in CD4+ cells infected with human herpes virus 6 and 7.

Requested From: United States
Date Requested: 12/4/2016

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