(tested or 100% immunogen sequence identity)
Protein G purified
- Immunofluorescence (1:10 - 1:50)
- Western blot
- Flow Cytometry (1:10 - 1:50)
CCR5 antibody was raised against synthetic phospho peptide corresponding to the C-Terminal region of human CD195 conjugated to BSA. ( pSer349 ).
Specific for human CD195, a cell surface antigen of approximately 40kD, also known as CCR5. Has been demonstrated to exclusively recognize CD195 phosphorylated at Ser 349(1). Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes. Recent studies have shown that upon ligand stimulation, CD195 is rapidly phosphorylated within the C-terminal domain. Recognizes a band of approximately 40kD on Western blots of RBL-CCR5 cell lysates.
Western Blot: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10 mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
PBS, pH 7.4, 0.09% sodium azide.
For research use only.
This antibody carries the LSBio 100% Guarantee.