Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 11n21] (IgG2a) to Human CCR3
Mouse Monoclonal (IgG2a) to Human CCR3
IHC, IHC - Paraffin, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin (10 µg/ml)
Specificity and Use
CCR3 antibody was raised against l1.2 cells expressing human CCR3 (C-C Chemokine Receptor 3).
Recognizes human CCR3.
Immunohistochemistry: LS-B3049 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3049 was determined to be 10 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
CCR3 is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells.