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Anti-CCNH / Cyclin H Antibody (clone 1B8) IHC-plus™ LS-B6020

Note: This antibody replaces LS-C133020
Catalog Size Price
LS-B6020-50 50 µg Unavailable

Most Popular CCNH / Cyclin H Antibodies

Anti-CCNH / Cyclin H Antibody (clone 1B8) IHC-plus™ LS-B6020
Mouse Monoclonal [clone 1B8] (IgG2a,k) to Human CCNH / Cyclin H
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H Antibody (C-Terminus, clone Mo-1.1) LS-C193395
Mouse Monoclonal [clone Mo-1.1] (IgG2b) to Human CCNH / Cyclin H
Human, Mouse
IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-CCNH / Cyclin H Antibody (aa225-275) LS-C288464
Rabbit Polyclonal (IgG) to Human CCNH / Cyclin H
Human, Rat, Dog, Pig
Immunoprecipitation
Unconjugated
Anti-CCNH / Cyclin H Antibody LS-C331195
Rabbit Polyclonal (IgG) to Human CCNH / Cyclin H
Human
Western blot, Immunoprecipitation
Unconjugated
Immunofluorescence analysis of HeLa cells.
Anti-CCNH / Cyclin H Antibody (C-Terminus) LS-C358933
Rabbit Polyclonal to Human CCNH / Cyclin H
Human, Monkey, Mouse, Rat, Pig
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of Cyclin H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Mouse Monoclonal [clone 1B8] (IgG2a,k) to Human CCNH / Cyclin H
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CCNH / Cyclin H
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1B8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

CCNH / Cyclin H antibody was raised against cCNH (AAH05280, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human Cyclin H
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

About CCNH / Cyclin H

P51946 NM_001239 NP_001230.1

CCNH Antibody, Cyclin H Antibody, p34 Antibody, p37 Antibody, CAK Antibody, CAK complex subunit Antibody, Cyclin-H Antibody, MO15-associated protein Antibody

CCNH / Cyclin H belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1.

Immunohistochemistry

Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CCNH expression in HeLa cell lysate.
Western blot of CCNH expression in HeLa cell lysate.

Western blot

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.
Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/25/2018

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