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Catalog Number Size Price
LS-C19092-100 100 µg (1.17 mg/ml) $567 
CCNE2 / Cyclin E2 Antibody - Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Polyclonal Rabbit anti‑Mouse CCNE2 / Cyclin E2 Antibody (WB) LS‑C19092

Polyclonal Rabbit anti‑Mouse CCNE2 / Cyclin E2 Antibody (WB) LS‑C19092

Antibody:
CCNE2 / Cyclin E2 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C19092-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCNE2 / Cyclin E2 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin E2 antibody LS-C19092 is an unconjugated rabbit polyclonal antibody to Cyclin E2 (CCNE2) from mouse. It is reactive with human and mouse. Validated for ELISA, IP and WB.
Target
Mouse CCNE2 / Cyclin E2
Synonyms
CCNE2 | Cyclin E2 | CYCE2 | G1/S-specific cyclin-E2
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:20000 - 1:85000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNE2 / Cyclin E2
O96020 AF091433 AAC80528.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024