LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CCNE2 / Cyclin E2 Antibody (C‑Terminus) LS‑C19092
Cyclin E2 antibody LS-C19092 is an unconjugated rabbit polyclonal antibody to Cyclin E2 (CCNE2) from human, mouse and rabbit. Validated for ELISA and WB.
Catalog
Size
Price
LS-C19092-100
100 µg
Unavailable

Popular CCNE2 / Cyclin E2 Products

IHC of paraffin-embedded human thyroid gland tissue, using Cyclin E2 (Ab-392) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of Cyclin E2 (N387) pAb in paraffin-embedded human thyroid gland tissue.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
Western blot of Cyclin E2 expression in COLO cells
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human thyroid tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

Cyclin E2 antibody LS-C19092 is an unconjugated rabbit polyclonal antibody to Cyclin E2 (CCNE2) from human, mouse and rabbit. Validated for ELISA and WB.
About CCNE2 / Cyclin E2
CCNE2 / Cyclin E2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. O96020 AF091433 AAC80528.1

CCNE2 Antibody, Cyclin E2 Antibody, CYCE2 Antibody, G1/S-specific cyclin-E2 Antibody


Specifications

Target
Rabbit CCNE2 / Cyclin E2
Host
Rabbit
Reactivity
Rabbit, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:20000 - 1:85000)
Immunogen
Synthetic peptide from rabbit CCNE2 / Cyclin E2.
Epitope
C-Terminus
Specificity
Amino acids at the carboxyl terminus of the Cyclin E2 protein.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.
Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Western blot

Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.
Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Western blot

Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.
Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Western blot

Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.
Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.


Requested From: 
Date Requested: 6/20/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number