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Anti-CCNE2 / Cyclin E2 Antibody (C-Terminus) LS-C19092

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCNE2 / Cyclin E2 Antibodies

Anti-CCNE2 / Cyclin E2 Antibody (aa2-15) LS-C53339
Rabbit Polyclonal to Human CCNE2 / Cyclin E2
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rabbit CCNE2 / Cyclin E2
Rabbit, Human, Mouse
Western blot, ELISA
Unconjugated

Details

Rabbit CCNE2 / Cyclin E2
Rabbit
Rabbit, Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:2000)
  • ELISA (1:20000 - 1:85000)

Specificity and Use

CCNE2 / Cyclin E2 antibody was raised against synthetic peptide from rabbit CCNE2 / Cyclin E2.
C-Terminus
Amino acids at the carboxyl terminus of the Cyclin E2 protein.
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About CCNE2 / Cyclin E2

O96020 AF091433 AAC80528.1

CCNE2 Antibody, Cyclin E2 Antibody, CYCE2 Antibody, G1/S-specific cyclin-E2 Antibody

CCNE2 / Cyclin E2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2.

Western blot

Western blot
Anti-Cyclin E2 Antibody - Western Blot. Western blot of affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.
 

Requested From: United States
Date Requested: 12/6/2016

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