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Anti-CCNE1 / Cyclin E1 Antibody LS-C149920

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCNE1 / Cyclin E1 Antibodies

Anti-CCNE1 / Cyclin E1 Antibody (clone HE12) LS-C88538
Mouse Monoclonal [clone HE12] (IgG1) to Human CCNE1 / Cyclin E1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human CCNE1 / Cyclin E1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CCNE1 / Cyclin E1
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

CCNE1 / Cyclin E1 antibody was raised against e. coli-derived recombinant human Cyclin E1 Met16-Lys 143 Accession # P24864.
Detects human Cyclin E1 in direct ELISAs and Western blots.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About CCNE1 / Cyclin E1

P24864 NM_001238 NP_001229.1

CCNE1 Antibody, Cyclin E1 Antibody, CCNE Antibody, Cyclin Es Antibody, Cyclin Et Antibody, G1/S-specific cyclin-E1 Antibody, G1/s-specific cyclin e Antibody, Cyclin E Antibody

CCNE1 / Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.

Immunohistochemistry

Immunohistochemistry
Cyclin E1 in Human Breast. Cyclin E1 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.

Western blot

Western blot
Detection of Human Cyclin E1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293 human embryonic kidney cell line. Gels were loaded with 25 ug of cytoplasmic (Cyto) and 25 ug of nuclear (Nuc) extracts. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for Cyclin E1 at approximately 50 - 60 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/8/2016

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