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Anti-CCNB1 / Cyclin B1 Antibody (phospho-Ser35) LS-C157610

Catalog Size Price
LS-C157610-400 400 µl Unavailable

Most Popular CCNB1 / Cyclin B1 Antibodies

Anti-CCNB1 / Cyclin B1 Antibody (Azide-free) LS-C22501
Mouse Monoclonal (IgG2a) to Human CCNB1 / Cyclin B1
Human, Mouse, Rat, Hamster
IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Unconjugated, Azide-free
Anti-CCNB1 / Cyclin B1 Antibody (phospho-Ser133) LS-C64078
Rabbit Monoclonal (IgG) to Human CCNB1 / Cyclin B1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-CCNB1 / Cyclin B1 Antibody (aa91-140) LS-C117561
Rabbit Polyclonal (IgG) to Human CCNB1 / Cyclin B1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CCNB1 / Cyclin B1 Antibody (clone V152) IHC-plus™ LS-B8608
Mouse Monoclonal [clone V152] (IgG1) to Hamster CCNB1 / Cyclin B1
Hamster, Human, Mouse, Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CCNB1 / Cyclin B1 Antibody (aa145-149) LS-C205411
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human
Dot Blot
Unconjugated

Details

Human CCNB1 / Cyclin B1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

Dot Blot (1:500)

Specificity and Use

pSer35
This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CCNB1 / Cyclin B1

P14635 NM_031966 NP_114172.1

CCNB1 Antibody, CCNB Antibody, Cyclin B1 Antibody, G2/mitotic-specific cyclin-B1 Antibody, G2/mitotic-specific cyclin B1 Antibody

CCNB1 / Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Western blot

Western blot
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot Blot
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Requested From: 
Date Requested: 8/21/2017

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