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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNB1 / Cyclin B1 Antibody (phospho‑Ser35) LS‑C157610
Cyclin B1 antibody LS-C157610 is an unconjugated rabbit polyclonal antibody to human Cyclin B1 (CCNB1). Validated for DB.
Catalog
Size
Price
LS-C157610-400
400 µl
Unavailable

Popular CCNB1 / Cyclin B1 Products

Antibody
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation
IHC of paraffin-embedded human breast carcinoma tissue, using Cyclin B1 (Ab-126) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-Cyclin B1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8608 dilution 10 ug/ml.
Species: Hamster, Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (aa145-149) antibody (left) or the same antibody preincubated with blocking peptide (right).
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

Cyclin B1 antibody LS-C157610 is an unconjugated rabbit polyclonal antibody to human Cyclin B1 (CCNB1). Validated for DB.
About CCNB1 / Cyclin B1
CCNB1 / Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. P14635 NM_031966 NP_114172.1

CCNB1 Antibody, CCNB Antibody, Cyclin B1 Antibody, G2/mitotic-specific cyclin-B1 Antibody, G2/mitotic-specific cyclin B1 Antibody


Specifications

Target
Human CCNB1 / Cyclin B1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Dot Blot (1:500)
Epitope
pSer35
Specificity
This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Western blot

Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Western blot

Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Western blot

Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.


Requested From: 
Date Requested: 6/21/2018

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