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Anti-CCNB1 / Cyclin B1 Antibody (phospho-Ser35) LS-C157610

Catalog Size Price
LS-C157610-400 400 µl Unavailable

Most Popular CCNB1 / Cyclin B1 Antibodies

Anti-CCNB1 / Cyclin B1 Antibody (Azide-free) LS-C22501
Mouse Monoclonal (IgG2a) to Human CCNB1 / Cyclin B1
Human, Mouse, Rat, Hamster
IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Unconjugated, Azide-free
Anti-CCNB1 / Cyclin B1 Antibody (phospho-Ser133) LS-C64078
Rabbit Monoclonal (IgG) to Human CCNB1 / Cyclin B1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-CCNB1 / Cyclin B1 Antibody (aa91-140) LS-C117561
Rabbit Polyclonal (IgG) to Human CCNB1 / Cyclin B1
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
IHC of paraffin-embedded human breast carcinoma tissue, using Cyclin B1 (Ab-126) Antibody. The picture on the right is treated with the synthesized peptide.
Anti-CCNB1 / Cyclin B1 Antibody (clone V152) IHC-plus™ LS-B8608
Mouse Monoclonal [clone V152] (IgG1) to Hamster CCNB1 / Cyclin B1
Hamster, Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Anti-Cyclin B1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8608 dilution 10 ug/ml.
Anti-CCNB1 / Cyclin B1 Antibody (aa145-149) LS-C205411
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (aa145-149) antibody (left) or the same antibody preincubated with blocking peptide (right).

100% Guaranteed
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human
Dot Blot
Unconjugated

Details

Human CCNB1 / Cyclin B1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

Dot Blot (1:500)

Specificity and Use

pSer35
This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CCNB1 / Cyclin B1

P14635 NM_031966 NP_114172.1

CCNB1 Antibody, CCNB Antibody, Cyclin B1 Antibody, G2/mitotic-specific cyclin-B1 Antibody, G2/mitotic-specific cyclin B1 Antibody

CCNB1 / Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Western blot

Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot Blot

Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Requested From: 
Date Requested: 4/25/2018

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