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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNA2 / Cyclin A2 Antibody (aa417‑432) LS‑C22475
Antibody
Cyclin A2 antibody LS-C22475 is an unconjugated rabbit polyclonal antibody to human Cyclin A2 (CCNA2). Validated for Func, ICC, IP and WB.
Catalog
Size
Price
LS-C22475-200
200 µg
Unavailable

Popular CCNA2 / Cyclin A2 Products

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Guinea pig, Pig, Rabbit, Zebrafish
Applications: Western blot
Anti-CCNA2 / Cyclin A2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8039 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Bovine, Guinea pig, Pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CCNA2 mAb.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Immunohistochemistry of paraffin-embedded mouse kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Antibody
Species: Human, Mouse, Rat, Sheep
Applications: ICC, Western blot, Immunoprecipitation

Product Description

Cyclin A2 antibody LS-C22475 is an unconjugated rabbit polyclonal antibody to human Cyclin A2 (CCNA2). Validated for Func, ICC, IP and WB.
About CCNA2 / Cyclin A2
CCNA2 / Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. P20248 NM_001237 NP_001228.1

CCNA2 Antibody, Cyclin-A2 Antibody, Cyclin A2 Antibody, CCN1 Antibody, CCNA Antibody, Cyclin-A Antibody, G2/mitotic-specific cyclin a Antibody


Specifications

Target
Human CCNA2 / Cyclin A2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation (1 - 5 µg/ml)
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide (KLH-coupled) corresponding to aa 417-432 (C-KHGVSLLNPPETLNL) within the C-terminus of human p58 cyclin A. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (93%); Hamster, Elephant, Panda, Dog, Bovine (87%); Rat, Horse, Platypus (80%).
Epitope
aa417-432
Specificity
Recognizes human p58 cyclin A. Does not cross-react with cyclin B or cyclin D. Species cross-reactivity: Mouse, rat and sheep.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 1-5 ug/ml. The precipitate may be used to perform a histone H1 kinase assay. Immunocytochemistry: 10 ug/ml.
Presentation
Lyophilized.
Reconstitution
200 µl deionized water
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/21/2018

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