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Anti-CCN3 / NOV Antibody LS-C104557

Note: This antibody replaces LS-C56929, LS-C37777

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCN3 / NOV Antibodies

Anti-CCN3 / NOV Antibody (C-Terminus) IHC-plus™ LS-B1831
Rabbit Polyclonal to Human CCN3 / NOV
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CCN3 / NOV Antibody (clone 2G8) IHC-plus™ LS-B6407
Mouse Monoclonal [clone 2G8] (IgG2a,k) to Human CCN3 / NOV
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CCN3 / NOV Antibody LS-C408821
Rabbit Polyclonal (IgG) to Human CCN3 / NOV
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CCN3 / NOV
Human
IHC - Paraffin, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human CCN3 / NOV
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (0.25 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

CCN3 / NOV antibody was raised against e.coli derived recombinant Human NOV.
Human NOV
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml-0.50 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hNOV by sandwich ELISA (using 100 &#956.l/well antibody solution) a concentration of 0.5-2 &#956.g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NOV (LS-C104549) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CCN3 / NOV

P48745 NM_002514 NP_002505.1

NOV Antibody, CCN3 Antibody, CCN family member 3 Antibody, IGFBP9 Antibody, IBP-9 Antibody, IGFBP-9 Antibody, NOVh Antibody, IGF-binding protein 9 Antibody, Nephroblastoma overexpressed Antibody, Protein NOV homolog Antibody

CCN3 / NOV is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.

Immunohistochemistry

Immunohistochemistry
IHC of CCN3 / NOV antibody LS-C104557. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of CCN3 / NOV antibody LS-C104557. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of CCN3 / NOV antibody LS-C104557. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of CCN3 / NOV antibody LS-C104557. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of CCN3 / NOV antibody LS-C104557. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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