Human CCL5 / RANTES

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Purified

Unmodified

Recombinant human RANTES.

Recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells. RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

• IHC - Paraffin
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

ELISA: May be used in an indirect ELISA or as a capture reagent in a sandwich ELISA. Western Blot: May be used in either reducing or non-reducing conditions.

PBS

Distilled Water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add azide.

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13501 NCBI: NM_002985 NP_002976.2