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Anti-CCL20 / MIP-3-Alpha Antibody IHC-plus™ LS-B7409

Note: This antibody replaces LS-C104419

Ordering

Wt. Vol. Conc. Price
500 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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CCL20 / MIP-3-Alpha Antibody LS-B7409 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CCL20 antibody IHC of human spleen, lymphocytes.

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Human CCL20 / MIP-3-Alpha
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CCL20 / MIP-3-Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Protein A purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (0.2 - 0.4 µg/ml), ELISA (2 - 4 µg/ml)

Specificity and Use

CCL20 / MIP-3-Alpha antibody was raised against e.coli derived recombinant Human MIP-3alpha.
Human MIP-3alpha
Immunohistochemistry: LS-B7409 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.

Packaging

Lyophilized from PBS. Sourced in TCS.
500 ul sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CCL20 / MIP-3-Alpha

P78556 NM_004591 NP_004582.1

CCL20 Antibody, Beta chemokine exodus-1 Antibody, Beta-chemokine exodus-1 Antibody, CKb4 Antibody, Exodus-1 Antibody, MIP-3a Antibody, MIP-3-alpha Antibody, LARC Antibody, ST38 Antibody, SCYA20 Antibody, C-C motif chemokine 20 Antibody, CC chemokine LARC Antibody, Mip-3alpha Antibody, MIP3A Antibody, Small-inducible cytokine A20 Antibody

Chemokines are a family of related proteins that regulate leukocyte migration (including into tissues) and activation in the face of inflammatory stimulus. CCL20/LARC/EXODUS/MIP-3 has been shown to be highly expressed in liver, and preferentially in lymphocytes and monocytes where its expression is upregulated by mediators of inflammation.

Immunohistochemistry

Immunohistochemistry
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7409 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 6/2/2015

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