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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCL2 / MCP1 Antibody (Internal, DY488) LS‑C142564
MCP1 antibody LS-C142564 is a DY488-conjugated rabbit polyclonal antibody to MCP1 (CCL2) from mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C142564-100
100 µl
Unavailable

Popular CCL2 / MCP1 Products

Antibody
Species: Rat
Applications: Western blot, ELISA, Neutralization
Western Blot (non-reducing) of CCL2 / MCP-1 antibody LS-C104751.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Anti-Human MCP-1 Antibody - Western Blot. Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E. coli. Recombinant (0.1 ug, 8.6 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Species: Human
Applications: Western blot, ELISA
IHC of paraffin-embedded liver cancer tissues using CCL2 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

MCP1 antibody LS-C142564 is a DY488-conjugated rabbit polyclonal antibody to MCP1 (CCL2) from mouse and rat. Validated for WB.
About CCL2 / MCP1
CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody


Specifications

Target
Rat CCL2 / MCP1
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CCL2 / MCP1 antibody was raised against synthetic peptide made to an internal portion of the rat protein (within residues 15-40). [Swiss-Prot# P14844]. Percent identity by BLAST analysis: Mouse (100%).
Epitope
Internal
Specificity
MCP1.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCP-1 Antibody - Western Blot on TNF alpha treated PC12 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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