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| Catalog Number | Size | Price |
|---|---|---|
| LS-C104751-50 | 50 µg | $356 |
| LS-C104751-100 | 100 µg | $396 |
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Polyclonal Rabbit anti‑Rat CCL2 / MCP1 Antibody (WB) LS‑C104751
Polyclonal Rabbit anti‑Rat CCL2 / MCP1 Antibody (WB) LS‑C104751
Note: This antibody replaces LS-C54182, LS-C8624, LS-C16449, LS-C42298
Antibody:
CCL2 / MCP1 Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CCL2 / MCP1 Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
MCP1 antibody LS-C104751 is an unconjugated rabbit polyclonal antibody to rat MCP1 (CCL2). Validated for ELISA and WB. Cited in 1 publication.
Target
Rat CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Host
Rabbit
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated.
Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Rat MCP-1.
Specificity
Rat MCP-1
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4% PFA. The primary antibody was incubated at 1 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/ml), a concentration of 4-6 ug/ml of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CCL2 / MCP1
LSBio Ratings
CCL2 / MCP1 Antibody for WB/Western, ELISA LS-C104751 has an LSBio Rating of
Publications (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Renal denervation and CD161a immune ablation prevent cholinergic hypertension and renal sodium retention. Nandita Raikwar, Cameron Braverman, Peter M Snyder, Robert A Fenton, David K Meyerholz, Francois M Abboud, Sailesh C Harwani. American journal of physiology. Heart and circulatory physiology. 2019 September;317:H517-H530.
Customer Reviews (0)
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Human, Monkey, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation, ELISA
Reactivity:
Mouse, Rat
Range:
31.2-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
