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Anti-CCL2 / MCP1 Antibody LS-C154026

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL2 / MCP1 Antibodies

Anti-CCL2 / MCP1 Antibody (aa102-130, clone ECE.2) LS-C139852
Rat Monoclonal [clone ECE.2] (IgG1) to Mouse CCL2 / MCP1
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-CCL2 / MCP1 Antibody (clone 2D8) LS-C169178
Mouse Monoclonal [clone 2D8] (IgG1) to Human CCL2 / MCP1
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Bovine CCL2 / MCP1
Bovine, Human, Mouse, Cat, Dog, Pig
Western blot, ELISA
Unconjugated

Details

Bovine CCL2 / MCP1
Rabbit
Bovine, Human, Mouse, Cat, Dog, Pig (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1 µg/ml)
  • ELISA

Specificity and Use

CCL2 / MCP1 antibody was raised against recombinant protein produced in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against swine based on 98% homology and human and cat (90%), orangutan and dog (89%) and mouse 90%). Cross-reactivity with CCL2 from other sources has not been determined.
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kD in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
100 µl Reconstitute with 100ul sterile ddH2O
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About CCL2 / MCP1

P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody

CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.

Western blot

Western blot
Anti-Bovine CCL2 Antibody - Western Blot. Western blot of protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kD (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Inc. Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (.
 

Requested From: United States
Date Requested: 12/9/2016

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