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Anti-CCL2 / MCP1 Antibody (Biotin) LS-C104657

Note: This antibody replaces LS-C54181, LS-C42982, LS-C16439

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL2 / MCP1 Antibodies

Anti-CCL2 / MCP1 Antibody (aa102-130, clone ECE.2) LS-C139852
Rat Monoclonal [clone ECE.2] (IgG1) to Mouse CCL2 / MCP1
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-CCL2 / MCP1 Antibody (clone 2D8) LS-C169178
Mouse Monoclonal [clone 2D8] (IgG1) to Human CCL2 / MCP1
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CCL2 / MCP1
Human
Western blot, ELISA
Biotin Conjugated

Details

Human CCL2 / MCP1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

CCL2 / MCP1 antibody was raised against e.coli derived recombinant Human MCP-1/MCAF.
Human MCP-1 /MCAF
ELISA: To detect hMCP-1/MCAF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-1/MCAF. To detect hMCP-1/MCAF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MCP-1/MCAF (LS-C104658) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-1/MCAF. Western Blot: To detect hMCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1/MCAF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CCL2 / MCP1

P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody

CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of CCL2 / MCP-1 antibody LS-C104657 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of CCL2 / MCP-1 antibody LS-C104657 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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