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Anti-CCL13 / MCP4 Antibody (clone 73506) LS-C72307


Wt. Vol. Conc. Price
500 µg 500 µl - Unavailable

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 73506] (IgG1) to Human CCL13 / MCP4
Flow Cytometry, ELISA, Functional Assay, Neutralization


Human CCL13 / MCP4
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [73506]
Protein G purified


  • Flow Cytometry
  • Functional Assay
  • Neutralization

Specificity and Use

CCL13 / MCP4 antibody was raised against e. coli-derived rhCCL13.
Recognizes human CCL13. In sandwich ELISAs, less than 1% cross-reactivity was observed with rhMCP-1 and no significant cross-reactivity or interference was observed with rhMCP-2, rhMCP-3, rmMARC, rmMCP-5, rmJE, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES or rmRANTES.
Neutralization of Bioactivity, Flow Cytometry, ELISA capture. Neutralization of Human CCL13 Bioactivity: The exact concentration of antibody required to neutralize human CCL13 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Flow Cytometry: This antibody was validated for flow cytometry using IL-1 treated A549 cells. For intracellular staining to detect human CCL13, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. ELISA Capture: This antibody can be used as a capture antibody in a human CCL13 ELISA in combination with the biotinylated polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 ul/well of the capture antibody at 2 ug/mL, in combination with 100 ul/well of the detection antibody, an ELISA for sample volumes of 100 ul can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 0.5 ng/ml.


Lyophilized from PBS, 5% trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 ml of PBS is used, the antibody concentration will be 500 ug/ml.
500 µl PBS
For research use only.

About CCL13 / MCP4

Q99616 NM_005408 NP_005399.1

CCL13 Antibody, C-C motif chemokine 13 Antibody, CKb10 Antibody, MCP-4 Antibody, MCP4 Antibody, Monocyte chemotactic protein 4 Antibody, New CC chemokine 1 Antibody, Small-inducible cytokine A13 Antibody, NCC1 Antibody, CK-beta-10 Antibody, NCC-1 Antibody, SCYA13 Antibody

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Requested From: 
Date Requested: 4/24/2017

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