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Anti-Ccl12 / MCP5 Antibody LS-C104804

Note: This antibody replaces LS-C54196, LS-C42255, LS-C25493

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Ccl12 / MCP5 Antibodies

Anti-Ccl12 / MCP5 Antibody (aa28-104) LS-C295408
Rabbit Polyclonal (IgG) to Mouse Ccl12 / MCP5
Mouse
Western blot, ELISA
Unconjugated
Western blot Image
Anti-Ccl12 / MCP5 Antibody (aa28-104, Biotin) LS-C301266
Rabbit Polyclonal (IgG) to Mouse Ccl12 / MCP5
Mouse
Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-Ccl12 / MCP5 Antibody (aa28-104, FITC) LS-C304052
Rabbit Polyclonal (IgG) to Mouse Ccl12 / MCP5
Mouse
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse Ccl12 / MCP5
Mouse
Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Mouse Ccl12 / MCP5
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (2 - 3 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Ccl12 / MCP5 antibody was raised against e.coli derived recombinant Murine MCP-5.
Mouse MCP-5
Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mMCP-5 (1000 ng/ml), a concentration of 2-3 ug/ml of this antibody is required. ELISA: To detect mMCP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MCP-5 (LS-C104806) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About Ccl12 / MCP5

Q62401 NM_011331 NP_035461.2

Ccl12 Antibody, MCP-5 Antibody, MCP5 Antibody, Scya12 Antibody

Chemokine (C-C motif) ligand 12 (Ccl12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine.

Western blot

Western blot
Western Blot (non-reducing) of MCP5 / CCL12 antibody LS-C104804. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of MCP5 / CCL12 antibody LS-C104804. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of MCP5 / CCL12 antibody LS-C104804. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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