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Catalog Number Size Price
LS-B2394-50 50 µg (0.5 mg/ml) $485 
CBX5 / HP1 Alpha Antibody - Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CBX5 / HP1 Alpha Antibody - CBX5 / HP1 Alpha antibody (0.1µg/ml) staining of K562 (A), MCF7 (B) and NIH3T3 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CBX5 / HP1 Alpha Antibody - Antibody (0.03 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CBX5 / HP1 Alpha Antibody - CBX5 / HP1 Alpha antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CBX5 / HP1 Alpha Antibody - Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CBX5 / HP1 Alpha Antibody - CBX5 / HP1 Alpha antibody (0.1µg/ml) staining of K562 (A), MCF7 (B) and NIH3T3 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CBX5 / HP1 Alpha Antibody - Antibody (0.03 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CBX5 / HP1 Alpha Antibody - CBX5 / HP1 Alpha antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human CBX5 / HP1 Alpha Antibody (aa88‑102, IHC, WB) LS‑B2394

IHC‑plus™ Polyclonal Goat anti‑Human CBX5 / HP1 Alpha Antibody (aa88‑102, IHC, WB) LS‑B2394

Note: This antibody replaces LS-C54619, LS-C67842
Antibody:
CBX5 / HP1 Alpha Goat anti-Human Polyclonal (aa88-102) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2394-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CBX5 / HP1 Alpha Goat anti-Human Polyclonal (aa88-102) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
HP1 Alpha antibody LS-B2394 is an unconjugated goat polyclonal antibody to HP1 Alpha (CBX5) (aa88-102) from human. It is reactive with human, bovine, horse and other species. Validated for Flow, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CBX5 / HP1 Alpha
Synonyms
CBX5 | Chromobox homolog 5 | HP1Hs alpha | HP1Hs-alpha | HP1 | HP1 alpha homolog | HP1 alpha | HP1-ALPHA | HP1A | Antigen p25 | Chromobox protein homolog 5
Host
Goat
Reactivity
Human, Monkey, Bovine, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1NP_001120793.1NP_001120794.1.
Epitope
aa88-102
Specificity
Human CBX5 / HP1. Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Flow Cytometry (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2394 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2394 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CBX5 / HP1 Alpha
P45973 NM_012117 NP_036249.1

Validation

CBX5 / HP1 Alpha Antibody - Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CBX5 / HP1 Alpha Antibody (aa88-102) for IHC, WB/Western, Flow LS-B2394 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024