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Catalog Number Size Price
LS-B128-50 50 µg (1.36 mg/ml) $515 
CBX4 Antibody - Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CBX4 Antibody - Anti-hPC2 Antibody - Immunofluorescence Microscopy. anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below). IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue). Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
CBX4 Antibody - Anti-hPC2 Antibody - Western Blot. Analysis shows the detection of human PC2 in probed lysates using Affinity Purified anti-hPC2 antibody.
CBX4 Antibody - Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CBX4 Antibody - Anti-hPC2 Antibody - Immunofluorescence Microscopy. anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below). IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue). Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
CBX4 Antibody - Anti-hPC2 Antibody - Western Blot. Analysis shows the detection of human PC2 in probed lysates using Affinity Purified anti-hPC2 antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CBX4 Antibody (IHC, IF, WB) LS‑B128

IHC‑plus™ Polyclonal Rabbit anti‑Human CBX4 Antibody (IHC, IF, WB) LS‑B128

Note: This antibody replaces LS-C18936, LS-C92015
Antibody:
CBX4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B128-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CBX4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CBX4 antibody LS-B128 is an unconjugated rabbit polyclonal antibody to human CBX4. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CBX4
Synonyms
CBX4 | Chromobox homolog 4 | Chromobox protein homolog 4 | E3 SUMO-protein ligase CBX4 | NBP16 | NS5ATP1-binding protein 16 | HPC2 | Polycomb 2 homolog | PC2 | Pc class 2 homolog
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (1:100 - 1:400)
  • Western blot (1:1000 - 1:4000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B128 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B128 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CBX4
O00257 NM_003655 NP_003646.2

Validation

CBX4 Antibody - Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CBX4 Antibody for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B128 has an LSBio Rating of
Laboratory Validation Score (4)

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024