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Anti-CBX4 Antibody (aa95-107) IHC-plus™ LS-B128

Note: This antibody replaces LS-C18936, LS-C92015


Wt. Vol. Conc. Price
50 µg - 1.36 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CBX4 Antibodies

Anti-CBX4 Antibody (N-Terminus) LS-C153640
Rabbit Polyclonal to Human CBX4
Western blot
Immunofluorescence Image
Anti-CBX4 Antibody IHC-plus™ LS-B8138
Rabbit Polyclonal (IgG) to Human CBX4
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human CBX4
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human CBX4
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (1:100 - 1:400)
  • Western blot (1:1000 - 1:4000)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

CBX4 antibody was raised against synthetic peptide from human CBX4.
aa 95-107 of Human PC2 protein.
Immunohistochemistry: LS-B128 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B128 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CBX4

O00257 NM_003655 NP_003646.2

CBX4 Antibody, Chromobox homolog 4 Antibody, Chromobox protein homolog 4 Antibody, E3 SUMO-protein ligase CBX4 Antibody, NBP16 Antibody, NS5ATP1-binding protein 16 Antibody, HPC2 Antibody, Polycomb 2 homolog Antibody, PC2 Antibody, Pc class 2 homolog Antibody

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development.


Anti-CBX4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B128 concentration 5 ug/ml.


Anti-hPC2 Antibody - Immunofluorescence Microscopy. anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below). IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue). Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Western blot

Western blot
Anti-hPC2 Antibody - Western Blot. Analysis shows the detection of human PC2 in probed lysates using Affinity Purified anti-hPC2 antibody.

Requested From: United States
Date Requested: 10/24/2016

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