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Anti-CBR3 Antibody (clone AT3C5) IHC-plus™ LS-B3589

Note: This antibody replaces LS-C103497

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

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Most Popular CBR3 Antibodies

Anti-CBR3 Antibody (clone AT3C5) IHC-plus™ LS-B3589
Mouse Monoclonal [clone AT3C5] (IgG1,k) to Human CBR3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CBR3 Antibody (clone 1G6) IHC-plus™ LS-B10988
Mouse Monoclonal [clone 1G6] (IgG1) to Human CBR3
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AT3C5] (IgG1,k) to Human CBR3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CBR3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AT3C5]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

CBR3 antibody was raised against recombinant CBR3 (1-277aa) purified from E. coli
CBR3 (1-277aa)
Immunohistochemistry: LS-B3589 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3589 was determined to be 5 ug/ml. Western Blot: Cell lysates of HepG2 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CBR3 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About CBR3

O75828 NM_001236 NP_001227.1

CBR3 Antibody, Carbonyl reductase [NADPH] 3 Antibody, Carbonyl reductase (NADPH) 3 Antibody, Carbonyl reductase 3 Antibody, HCBR3 Antibody, SDR21C2 Antibody

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1.

Immunohistochemistry

Immunohistochemistry
Anti-CBR3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3589 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CBR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3589 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CBR3 antibody IHC of human brain, cortex, white matter. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3589 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of HepG2 cell lysate.

Requested From: United States
Date Requested: 12/6/2016

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