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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBLN1 / Cerebellin 1 Antibody IHC‑plus™ LS‑B478
Note: This antibody replaces LS-C19529, LS-C6054
Cerebellin 1 antibody LS-B478 is an unconjugated rabbit polyclonal antibody to Cerebellin 1 (CBLN1) from human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B478-50
50 µg (1 mg/ml)
Unavailable

Popular CBLN1 / Cerebellin 1 Products

Immunocytochemistry of precerebellin in 3T3 cells with precerebellin antibody at 10 µg/mL.
Species: Human, Mouse, Rat
Applications: ICC, Western blot
Western blot of extracts from HUVEC cells, using CBLN1 Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of CBLN1 / Cerebellin 1 antibody.
Species: Human
Applications: IHC, Western blot
Western blot of Cerebellin 1 antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

Cerebellin 1 antibody LS-B478 is an unconjugated rabbit polyclonal antibody to Cerebellin 1 (CBLN1) from human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About CBLN1 / Cerebellin 1
Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. P23435 NM_004352 NP_004343.1

CBLN1 Antibody, CLN1 Antibody, Cerebellin Antibody, Cerebellin 1 precursor Antibody, Precerebellin Antibody, Cerebellin-1 Antibody


Specifications

Target
Human CBLN1 / Cerebellin 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (2 - 4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30168 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B478. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Immunohistochemistry: LS-B478 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B478 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats. Dmitrieff EF, Wilson JT, Dunmire KB, Bavis RW. Respiratory Physiology & Neurobiology. 2011 175:220-7. (IHC-Fr; Rat) [Full Text Article] [PubMed:21094282] [PMC:PMC3033470]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Western blot

Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.

Western blot

Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.
Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Western blot

Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.

Western blot

Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.
Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Western blot

Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.

Western blot

Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.
Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Immunohistochemistry

Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.
Anti-CBLN1 / Precerebellin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B478 concentration 10 ug/ml.

Western blot

Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 µg/mL.

Western blot

Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.
Western blot of precerebellin in mouse cerebellum lysate with LS-B478 at (A) 2 and (B) 4 ug/ml.


Requested From: 
Date Requested: 6/24/2018

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