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Anti-CAV1 / Caveolin 1 Antibody LS-C149898

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular CAV1 / Caveolin 1 Antibodies

Anti-CAV1 / Caveolin 1 Antibody (aa73-84) IHC-plus™ LS-B192
Goat Polyclonal to Human CAV1 / Caveolin 1
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CAV1 / Caveolin 1 Antibody (N-Terminus) IHC-plus™ LS-B9776
Goat Polyclonal (IgG) to Human CAV1 / Caveolin 1
Human, Mouse, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human CAV1 / Caveolin 1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CAV1 / Caveolin 1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.2 µg/ml)

Specificity and Use

CAV1 / Caveolin 1 antibody was raised against e. coli-derived recombinant human Caveolin1 Ser2-Ser104 Accession # Q03135.
Detects human Caveolin1 in Western blots.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CAV1 / Caveolin 1

Q03135 NM_001753 NP_001744.2

CAV1 Antibody, Caveolin-1 Antibody, CGL3 Antibody, CAV Antibody, MSTP085 Antibody, VIP21 Antibody, BSCL3 Antibody

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade.

Immunohistochemistry

Immunohistochemistry
Caveolin-1 in Human Liver. Caveolin-1 was detected in immersion fixed paraffin-embedded sections of human liver using Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi.

Western blot

Western blot
Detection of Human Caveolin-1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.2 ug/ml of Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for Caveolin-1 at approximately 21 to 24 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 1/23/2017

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