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Anti-CASZ1 Antibody (Internal) LS-C154253

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CASZ1 Antibodies

Anti-CASZ1 Antibody (N-Terminus) IHC-plus™ LS-B2838
Rabbit Polyclonal to Human CASZ1
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CASZ1 Antibody (aa1574-1601, FITC) LS-C246979
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse
Western blot, ELISA
FITC Conjugated
Anti-CASZ1 Antibody (aa1574-1601, Azide-free, HRP) LS-C249575
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse, Drosophila, Macaque
Western blot, ELISA
Unconjugated

Details

Human CASZ1
Rabbit
Human, Mouse, Drosophila, Macaque (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:5000)
  • ELISA

Specificity and Use

CASZ1 antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Internal
A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
This antibody has been tested for use in ELISA and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kD for hCASZ5 and 190 kD for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CASZ1

Q86V15 NM_017766 NP_060236.3

CASZ1 Antibody, Castor-related protein Antibody, CAS11 Antibody, Castor Antibody, Castor homolog 1, zinc finger Antibody, CST Antibody, DJ734G22.1 Antibody, Zinc finger protein 693 Antibody, SRG Antibody, ZNF693 Antibody, Castor zinc finger 1 Antibody, Survival-related Antibody

Transcription factor involved in vascular assembly and morphogenesis through direct transcriptional regulation of EGFL7.

Western blot

Western blot
Anti-hCASZ1 Antibody - Western Blot. Western blot of anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kD). Lane 4, SY5Y transfected with hCASZ11 (190kD). Personal communication, Carol Thiele, NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/9/2016

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