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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASQ2 / Calsequestrin 2 Antibody (aa124‑388) LS‑C185748
Calsequestrin 2 antibody LS-C185748 is an unconjugated rabbit polyclonal antibody to human Calsequestrin 2 (CASQ2). Validated for IHC and WB.
Catalog
Size
Price
LS-C185748-100
100 µl (0.68 mg/ml)
Unavailable

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Product Description

Calsequestrin 2 antibody LS-C185748 is an unconjugated rabbit polyclonal antibody to human Calsequestrin 2 (CASQ2). Validated for IHC and WB.
About CASQ2 / Calsequestrin 2
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. O14958 NM_001232 NP_001223.2

CASQ2 Antibody, Calsequestrin-2 Antibody, PDIB2 Antibody, Calsequestrin 2 Antibody


Specifications

Target
Human CASQ2 / Calsequestrin 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 124 and 388 (O14958) of Calsequestrin 2. Percent identity by BLAST analysis: Human (100%); Rat, Rabbit (96%); Mouse, Bovine (95%).
Epitope
aa124-388
Specificity
Human Calsequestrin 2
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.

Western blot

Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.

Western blot

Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.

Western blot

Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.
IHC of paraffin-embedded Cal27 xenograft using Calsequestrin-2 antibody at 1:500 dilution.

Western blot

Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A: H1299. 10% SDS PAGE. Calsequestrin 2 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/22/2018

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