Human CASP9 / Caspase 9

Rabbit

Human, Mouse, Rat, Bovine, Sheep (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Recombinant human pro-caspase-9 protein, N-Terminus fragment (aa 1-134) with a C-Terminus histidine tag.

N-Terminus

Detects a ~46-50kD protein, corresponding to the apparent molecular mass of the proform of caspase-9 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine and sheep. In apoptotic cells, the antibody also detects 35/37kD, 18kD and 10kD proteins which correspond to processed forms of caspase-9. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis.

• Western blot

Western Blot (ECL): 1:2000 (human). Western Blot (ECL): 1:500 (other species). Positive control: HeLa Heat Shocked Cell Lysate.

Serum

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P55211 NCBI: NM_001229 NP_001220.2