Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human CASP9 / Caspase 9
Human, Mouse, Rat, Dog, Gerbil (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
CASP9 / Caspase 9 antibody was raised against recombinant full length human Caspase-9 protein (pro-form).
This polyclonal antisera recognizes the proform of caspase-9 (~50 kD), and the large (~35 kD) and small (~15 kD) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kD.
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
CASP9 / Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.
IHC of Caspase-9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections using LS-C148248 at 1:2000. Hematoxylin-Eosin counterstain. A: Brain striatum. B: Brain stem motor neuron.
Western blot of Caspase-9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 kD pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.
Requested From: United States
Date Requested: 2/27/2017